1921
Volume 69, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Microsatellite markers derived from simple sequence repeats have been useful in studying a number of human pathogens, including the human malaria parasite . Genetic markers for would likewise help elucidate the genetics and population characteristics of this other important human malaria parasite. We have identified a locus in a telomeric clone that contains simple sequence repeats. Primers were designed to amplify this region using a two-step semi-nested polymerase chain reaction protocol. The primers did not amplify template obtained from non-infected individuals, nor DNA from primates infected with the other human malaria parasites (, , or ). The marker was polymorphic in -infected field isolates obtained from Papua New Guinea, Indonesia and Guyana. This microsatellite marker may be useful in genetic and epidemiologic studies of malaria.

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2003-10-01
2017-11-22
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References

  1. Mendis K, Sina BJ, Marchesini P, Carter R, 2001. The neglected burden of Plasmodium vivax malaria. Am J Trop Med Hyg 64 (suppl) : 97–106.
  2. Baird JK, 2000. Resurgent malaria at the millennium: control strategies in crisis. Drugs 59 : 719–743.
  3. Su X, Kirkman LA, Fujioka H, Wellems TE, 1997. Complex polymorphisms in an approximately 330 kDa protein are linked to chloroquine-resistant P. falciparum in Southeast Asia and Africa. Cell 91 : 593–603.
  4. Su X, Ferdig MT, Huang Y, Huynh CQ, Liu A, You J, Wootton JC, Wellems TE, 1999. A genetic map and recombination parameters of the human malaria parasite Plasmodium falciparum. Science 286 : 1351–1353.
  5. Ferdig MT, Su XZ, 2000. Microsatellite markers and genetic mapping in Plasmodium falciparum. Parasitol Today 16 : 307–312.
  6. Bruce MC, Galinski MR, Barnwell JW, Snounou G, Day KP, 1999. Polymorphism at the merozoite surface protein-3alpha locus of Plasmodium vivax: global and local diversity. Am J Trop Med Hyg 61 : 518–525.
  7. Anderson TJ, Su XZ, Bockarie M, Lagog M, Day KP, 1999. Twelve microsatellite markers for characterization of Plasmodium falciparum from finger-prick blood samples. Parasitology 119 : 113–125.
  8. Anderson TJ, Su XZ, Roddam A, Day KP, 2000. Complex mutations in a high proportion of microsatellite loci from the protozoan parasite Plasmodium falciparum. Mol Ecol 9 : 1599–1608.
  9. Wootton JC, Feng X, Ferdig MT, Cooper RA, Mu J, Baruch DI, Magill AJ, Su XZ, 2002. Genetic diversity and chloroquine selective sweeps in Plasmodium falciparum. Nature 418 : 320–323.
  10. Carlton JM, Galinski MR, Barnwell JW, Dame JB, 1999. Karyotype and synteny among the chromosomes of all four species of human malaria parasite. Mol Biochem Parasitol 101 : 23–32.
  11. Carlton J, 2003. The Plasmodium vivax genome sequencing project. Trends Parasitol 19 : 227–231.
  12. Gardner MJ, Hall N, Fung E, White O, Berriman M, Hyman RW, Carlton JM, Pain A, Nelson KE, Bowman S, Paulsen IT, James K, Eisen JA, Rutherford K, Salzberg SL, Craig A, Kyes S, Chan MS, Nene V, Shallom SJ, Suh B, Peterson J, Angiuoli S, Pertea M, Allen J, Selengut J, Haft D, Mather MW, Vaidya AB, Martin DM, Fairlamb AH, Fraunholz MJ, Roos DS, Ralph SA, McFadden GI, Cummings LM, Subramanian GM, Mungall C, Venter JC, Carucci DJ, Hoffman SL, Newbold C, Davis RW, Fraser CM, Barrell B, 2002. Genome sequence of the human malaria parasite Plasmodium falciparum. Nature 419 : 498–511.
  13. Tchavtchitch M, Fischer K, Huestis R, Saul A, 2001. The sequence of a 200 kb portion of a Plasmodium vivax chromosome reveals a high degree of conservation with Plasmodium falciparum chromosome 3. Mol Biochem Parasitol 118 : 211–222.
  14. del Portillo HA, Fernandez-Becerra C, Bowman S, Oliver K, Preuss M, Sanchez CP, Schneider NK, Villalobos JM, Rajandream MA, Harris D, Pereira da Silva LH, Barrell B, Lanzer M, 2001. A superfamily of variant genes encoded in the sub-telomeric region of Plasmodium vivax. Nature 410 : 839–842.
  15. Carlton JM, Muller R, Yowell CA, Fluegge MR, Sturrock KA, Pritt JR, Vargas-Serrato E, Galinski MR, Barnwell JW, Mulder N, Kanapin A, Cawley SE, Hide WA, Dame JB, 2001. Profiling the malaria genome: a gene survey of three species of malaria parasite with comparison to other apicomplexan species. Mol Biochem Parasitol 118 : 201–210.
  16. Benson G, 1999. Tandem repeats finder: a program to analyze DNA sequences. Nucleic Acids Res 27 : 573–580.
  17. Mehlotra RK, Kasehagen LJ, Baisor M, Lorry K, Kazura JW, Bockarie MJ, Zimmerman PA, 2002. Malaria infections are randomly distributed in diverse holoendemic areas of Papua New Guinea. Am J Trop Med Hyg 67 : 555–562.
  18. Nei M, Kumar S, 2000. Molecular Evolution and Phylogenetics. Oxford: Oxford University Press.
  19. Sumawinata IW, Bernadeta, Leksana B, Sutamihardja A, Purnomo, Subianto B, Sekartuti, Fryauff DJ, Baird JK, 2003. Very high risk of therapeutic failure with chloroquine for uncomplicated Plasmodium falciparum and P. vivax malaria in Indonesian Papua. Am J Trop Med Hyg 69 : 416–420.
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  • Received : 12 May 2003
  • Accepted : 14 Jul 2003

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