Volume 69, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


We conducted a population genetic analysis of collected from 20 sites in Brazil, the United States (Florida, Georgia, and Illinois), and the Cayman Islands. Using isoenzyme analysis, we examined genetic diversity and patterns of gene flow. High genetic differentiation was found among Brazilian samples, and between them and North American samples. Regression analysis of genetic differentiation according to geographic distances indicated that samples from Florida were genetically isolated by distance. Infection rates with dengue and yellow fever viruses showed greater differences between two Brazilian samples than between the two North American samples or between a Brazilian sample and a North American sample. Introductions and establishments of new populations in the Americas are still in progress, shaping population genetic composition and potentially modifying both dengue and yellow fever transmission patterns.


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  1. Smith CEG, 1956. The history of dengue in tropical Asia and its probable relationship to the mosquito Aedes aegypti. J Trop Med Hyg 59 : 243–252.
  2. Rai KS, 1991. Aedes albopictus in the Americas. Annu Rev Entomol 36 : 459–484.
  3. Sprenger D, Wuithiranyagool T, 1986. The discovery and distribution of Aedes albopictus in Harris County, Texas. J Am Mosq Control Assoc 2 : 217–219.
  4. Hawley WA, Reiter P, Copeland RS, Pumpuni CB, Craig GB Jr, 1987. Aedes albopictus in North America: probable introduction in used tires from northern Asia. Science 236 : 1114–1116.
  5. Consoli RAGB, Lourenço-de-Oliveira R, 1994. Principais Mosquitos de Importância Sanitária no Brasil. Rio de Janeiro: Fiocruz.
  6. Nasci RS, 1995. Control of Aedes albopictus from the perspective of North America. Parasitologia 37 : 123–127.
  7. Mattingly PF, 1957. Genetical aspects of the Aedes aegypti problem. I. Taxonomy and bionomics. Ann Trop Med Parasitol 51 : 392–408.
  8. Lounibos LP, 2002. Invasion by insect vectors of human diseases. Annu Rev Entomol 47 : 233–266.
  9. O’Meara GF, Evans LF Jr, Gettman AD, Cuda JP, 1995. Spread of Aedes albopictus and decline of Ae. aegypti (Diptera: Culicidae) in Florida. J Med Entomol 32 : 554–562.
  10. Barrera R, 1996. Competition and resistance to starvation in larvae of container-inhabiting Aedes mosquitoes. Ecol Entomol 21 : 117–127.
  11. Lounibos LP, Escher RL, Nishimura N, Juliano SA, 1997. Long-term dynamics of a predator used for biological control and decoupling from mosquito prey in a subtropical treehole ecosystem. Oecologia 111 : 189–200.
  12. Rudnick A, 1965. Studies of the ecology of dengue in Malaysia: a preliminary report. J Med Entomol 2 : 203–208.
  13. Hawley WA, 1988. The biology of Aedes albopictus. J Am Mosq Control Assoc 4 (Suppl): 1–39.
  14. Knudsen AB, 1983. Aedes aegypti and dengue in the Caribbean. Mosq News 43 : 269–275.
  15. Brès PLJ, 1986. A century of progress in combating yellow fever. Bull Worls Health Organ 64 : 775–786.
  16. Robertson SE, Hull BP, Tomori O, Bele O, LeDuc JW, Esteves K, 1996. Yellow fever: a decade of reemergence. JAMA 276 : 1157–1162.
  17. Pinheiro FP, Corber SJ, 1997. Global situation of dengue and dengue haemorrhagic fever, and its emergence in the Americas. World Health Stat Q 50 : 161–169.
  18. Gubler DJ, Clark GG, 1995. Dengue/dengue hemorrhagic fever. The emergence of a global health problem. Emerg Infect Dis 1 : 55–57.
  19. van der Stuyft P, Gianella A, Pirard M, Cespedes J, Lora J, Peredo C, Pelegrino JL, Vorndam V, Boelaert M, 1999. Urbanization of yellow fever in Santa Cruz, Bolivia. Lancet 353 : 1558–1562.
  20. Prata A, 2000. Yellow fever. Mem Inst Oswaldo Cruz 95 (Suppl I): 183–187.
  21. Guzman MG, Kouri GP, Bravo J, Soler M, Vazquez S, Santos M, Villaescusa R, Basanta P, Indan G, Ballester JM, 1984. Dengue haemorrhagic fever in Cuba. II Clinical investigations. Trans R Soc Trop Med Hyg 78 : 239–241.
  22. Gubler DJ, 2002. Epidemic dengue/dengue hemorrhagic fever as a public health, social and economic problem in the 21st century. Trends Microbiol 10 : 100–103.
  23. Shroyer DA, 1986. Aedes albopictus and arboviruses: a concise review of the literature. J Am Mosq Control Assoc 2 : 424–428.
  24. Ibanez-Bernal S, Briseno B, Mutebi JP, Argot E, Rodriguez G, Martinez-Campos C, Paz R, De la Fuente-San Roman P, Tapia-Conyer R, Flisser A, 1997. First record in America of Aedes albopictus naturally infected with dengue virus during the 1995 outbreak at Reynosa, Mexico. Med Vet Entomol 11 : 305–309.
  25. Serufo JC, Montes de Oca H, Tavares VA, Souza AM, Rosa RV, Jamal MC, Lemos JR, Oliveira MA, Noguiera RMR, Schatzmayr HG, 1993. Isolation of dengue virus type 1 from larvae of Aedes albopictus in Campos Altos city, state of Minas Gerais, Brazil. Mem Inst Oswaldo Cruz 88 : 503–504.
  26. Rudnick A, Marchette NJ, Garcia R, 1967. Possible jungle dengue-recent studies and hypotheses. Jpn J Med Sci Biol 20 : 69–74.
  27. Pan American Health Organization, 1987. Control of Aedes albopictus in the Americas. Bull Pan Am Health Organ 21 : 314–324.
  28. Mitchell CJ, Miller BR, Gubler DJ, 1987. Vector competence of Aedes albopictus from Houston, Texas, for dengue serotypes 1 to 4, yellow fever and Ross River viruses. J Am Mosq Control Assoc 3 : 460–465.
  29. Boromisa RD, Rai KS, Grimstad PR, 1987. Variation in the vector competence of geographic strains of Aedes albopictus for dengue 1 virus. J Am Mosq Control Assoc 3 : 378–386.
  30. Reiter P, Amador M, Colon N, 1991. Enhancement of the CDC ovitrap with hay infusions for daily monitoring of Aedes aegypti populations. J Am Mosq Control Assoc 7 : 52–55.
  31. Vazeille-Falcoz M, Mousson L, Rodhain F, Chungue E, Failloux AB, 1999. Variation in oral susceptibility to dengue type 2 virus of populations of Aedes aegypti from the islands of Tahiti and Moorea, French Polynesia. Am J Trop Med Hyg 60 : 292–299.
  32. Lourenço de Oliveira R, Vazeille M, Bispo AM, Failloux AB, 2002. Oral susceptibility to yellow fever virus of Aedes aegypti from Brazil. Mem Inst Oswaldo Cruz 97 : 437–439.
  33. Kuberski TT, Rosen L, 1977. A simple technique for the detection of dengue antigen in mosquitoes by immunofluorescence. Am J Trop Med Hyg 26 : 533–537.
  34. Failloux AB, Vazeille M, Rodhain F, 2003. Geographic genetic variation in populations of the dengue virus vector Aedes aegypti. J Mol Evol 55 : 653–663.
  35. Raymond M, Rousset F, 1995. Genepop (version 1.2): population genetics software for exact tests and ecumenicism. J Hered 86 : 248–249.
  36. Weir BS, Cockerham CC, 1984. Estimating F-statistics for the analysis of population structure. Evolution 38 : 1358–1370.
  37. Rousset F, Raymond M, 1995. Testing heterozygote excess and deficiency. Genetics 140 : 1413–1419.
  38. Slatkin M, 1993. Isolation by distance in equilibrium and non-equilibrium populations. Evolution 47 : 264–279.
  39. Ohta T, 1982. Linkage disequilibrium due to random genetic drift in finite subdivided populations. Proc Natl Acad Sci USA 79 : 1940–1944.
  40. Hafkin B, Kaplan JE, Reed C, Elliott LB, Fontaine R, Sather GE, Kappus K, 1982. Reintroduction of dengue fever into the continental United States. I. Dengue surveillance in Texas, 1980. Am J Trop Med Hyg 31 : 1222–1228.
  41. Schatzmayr HG, 2000. Dengue situation in Brazil by year 2000. Mem Inst Oswaldo Cruz 95 : 179–181.
  42. Lorenz L, Beaty BJ, Aitken TH, Wallis GP, Tabachnick WJ, 1984. The effect of colonization upon Aedes aegypti susceptibility to oral infection with yellow fever virus. Am J Trop Med Hyg 33 : 690–694.
  43. Vazeille M, Rosen L, Mousson L, Failloux AB, 2003. Low oral receptivity for dengue type 2 viruses of Aedes albopictus from south-east Asia compared to that of Aedes aegypti. Am J Trop Med Hyg 68 : 203–208.
  44. Miller BR, Ballinger ME, 1988. Aedes albopictus mosquitoes introduced into Brazil: vector competence for yellow fever and dengue viruses. Trans R Soc Trop Med Hyg 82 : 476–477.
  45. Jupp PG, Kemp A, 2002. Laboratory vector competence experiments with yellow fever virus and five South African mosquito species including Aedes aegypti. Trans R Soc Trop Med Hyg 96 : 493–498.
  46. Ayres CFJ, Romao TPA, Melo-Santos MAV, Furtado AF, 2002. Genetic diversity in Brazilian populations of Aedes albopictus. Mem Inst Oswaldo Cruz 97 : 871–875.
  47. Black WCIV, Ferrari JA, Rai KS, Sprenger D, 1988. Breeding structure of a colonizing species: Aedes albopictus (Skuse) in the United States. Heredity 60 : 173–181.
  48. Gorrochotagui-Escalante N, De Lourdes Munoz M, Fernandez-Salas I, Beaty BJ, Black WC IV, 2000. Genetic isolation by distance among Aedes aegypti populations along the northeastern coast of Mexico. Am J Trop Med Hyg 62 : 200–209.
  49. Gorrochotagui-Escalante N, Gomez-Machorro C, Lozano-Fuentes S, Fernandez-Salas I, De Lourdes Munoz M, Farfan-Ale JA, Garcia-Rejon J, Beaty BJ, Black WC IV, 2002. Breeding structure of Aedes aegypti populations in Mexico varies by region. Am J Trop Med Hyg 66 : 213–222.
  50. Honório NA, Silva WC, Leite PJ, Gonçalves JM, Lounibos LP, Lourenço-de-Oliveira R, 2002. Dispersal of Aedes aegypti and Aedes albopictus (Diptera: Culicidae) in a urban endemic dengue area in the state of Rio de Janeiro, Brazil. Mem Inst Oswaldo Cruz 98 : 191–198.
  51. Vazeille M, Mousson L, Rakatoarivony I, Villeret R, Rodhain F, Duchemin JB, Failloux AB, 2001. Population genetic structure and competence as a vector for dengue 2 virus of Aedes aegypti and Aedes albopictus from Madagascar. Am J Trop Med Hyg 65 : 491–497.
  52. Birungi J, Munstermann LE, 2002. Genetic structure of Aedes albopictus (Diptera: Culicidae) populations based on mitochondrial ND5 sequences: evidence for an independent invasion into Brazil and United States. Ann Entomol Soc Am 95 : 125–132.
  53. Mitchell CJ, Miller BR, 1990. Vertical transmission of dengue viruses by strains of Aedes albopictus recently introduced into Brazil. J Am Mosq Control Assoc 6 : 251–253.

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  • Received : 14 Oct 2002
  • Accepted : 10 Mar 2003

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