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Volume 68, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645
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Abstract

Antibody-mediated inhibition of parasites reflects the potential parasite-neutralizing activity of the antibodies . In this study, immunoglobulins and isolates were collected from children with asymptomatic malaria in Burkina Faso. We demonstrate a significantly lower growth inhibitory activity against the field isolates by autologous host immunoglobulin compared with that of immunoglobulin from other individuals. To gain further insight to possible mechanisms for the diverse sensitivity observed, analyses of consecutive isolates taken 14 days apart were performed with regard to polymerase chain reaction–based genotyping and sensitivity to growth inhibition . All the asymptomatic infections were composed of multiple, genotypically distinct parasite clones, and at least one new parasite clone appeared in most of the day 14 isolates compared with the corresponding day 0 isolates. Apparently persisting parasite clones, present in both the day 0 and day 14 isolates from the same person, were also frequently observed. The day 14 isolates were more effectively inhibited by autologous day 14 immunoglobulin than by the corresponding day 0 immunoglobulin in 57% of the cases. However, the frequent presence of persisting parasite clones in asymptomatic children indicates that the parasite may develop a relative resistance to neutralizing immune responses.

Copyright 2003 The American Society of Tropical Medicine
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2003-06-01
2019-11-21

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References

  1. Greenwood BM, Bradley AK, Greenwood AM, Byass P, Jammeh K, Marsh K, Tulloch S, Oldfield FSJ, Hayes R, 1987. Mortality and morbidity from malaria among children in a rural area of The Gambia, West Africa. Trans R Soc Trop Med Hyg 81 : 478–486. [Google Scholar]
  2. Robert F, Ntoumi F, Angel G, Candito D, Rogier C, Fandeur T, Sarthou J-L, Mercereau-Puijalon O, 1996. Extensive genetic diversity of Plasmodium falciparum isolates collected from patients with severe malaria in Dakar, Senegal. Trans R Soc Trop Med Hyg 90 : 704–711. [Google Scholar]
  3. Daubersies P, Sallenave-Sales S, Magne S, Trape J-F, Contamin H, Fandeur T, Rogier C, Mercereau-Puijalon O, Druilhe P, 1996. Rapid turnover of Plasmodium falciparum populations in asymptomatic individuals living in a high transmission area. Am J Trop Med Hyg 54 : 18–26. [Google Scholar]
  4. Babiker HA, Abdel-Muhsin ABA, Ranford-Cartwright LC, Satti G, Walliker D, 1998. Characteristics of Plasmodium falciparum parasites that survive the lengthy dry season in eastern Sudan where malaria transmission is markedly seasonal. Am J Trop Med Hyg 59 : 582–590. [Google Scholar]
  5. Bolad A, Berzins K, 2000. Antigenic diversity of Plasmodium falciparum and antibody-mediated parasite neutralization. Scand J Immunol 52 : 233–239. [Google Scholar]
  6. Iqbal J, Siripoon N, Snounou G, Perlmann P, Berzins K, 1997. Plasmodium falciparum: selection of parasite subpopulations with decreased sensitivity for antibody-mediated growth inhibition in vitro. Parasitology 114 : 317–324. [Google Scholar]
  7. Wåulin Flyg M, Perlmann H, Perlmann P, Esposito F, Berzins K, 1997. Wild isolates of Plasmodium falciparum malaria show decreased sensitivity to in vitro inhibition of parasite growth mediated by autologous host antibodies. Clin Exp Immunol 107 : 321–327. [Google Scholar]
  8. Esposito F, Lombardi S, Modiano D, Zavala F, Reeme J, Lamizana L, Coluzzi M, Nussenzweig RS, 1988. Prevalence and levels of antibodies to the circumsporozoite protein of Plasmodium falciparum in an endemic area and their relationship to resistance against malaria infection. Trans R Soc Trop Med Hyg 82 : 827–832. [Google Scholar]
  9. Trager W, Jensen JB, 1976. Human malaria parasites in continuous culture. Science 193 : 673–675. [Google Scholar]
  10. Harboe N, Ingild A, 1973. Immunization, isolation of immunoglobulins, estimation of antibody titre. Scand J Immunol 2 (Suppl 1) : 161–164. [Google Scholar]
  11. Wåhlin B, Wahlgren M, Perlmann H, Berzins K, Björkman A, Patarroyo ME, Perlmann P, 1984. Human antibodies to a Mr 155,000 Plasmodium falciparum antigen efficiently inhibit merozoite invasion. Proc Natl Acad Sci USA 81 : 7912–7916. [Google Scholar]
  12. Cranmer SL, Magowan C, Liang J, Coppel RL, Cooke BM, 1997. An alternative to serum for cultivation of Plasmodium falciparum in vitro. Trans R Soc Trop Med Hyg 91 : 363–365. [Google Scholar]
  13. Troye-Blomberg M, Perlmann H, Patarroyo ME, Perlmann P, 1983. Regulation of the immune response in Plasmodium falciparum malaria II. Antigen specific proliferative responses in vitro. Clin Exp Immunol 53 : 345–353. [Google Scholar]
  14. Perlmann H, Helmby H, Hagstedt M, Carlson J, Larsson PH, Troye-Blomberg M, Perlmann P, 1994. IgE elevation and IgE anti-malarial antibodies in Plasmodium falciparum malaria: association of high IgE levels with cerebral malaria. Clin Exp Immunol 97 : 284–292. [Google Scholar]
  15. Snounou G, Viriyakosol S, Jarra W, Thaithong S, Brown KN, 1993. Identification of the four human malaria parasite species in field samples by the polymerase chain reaction and detection of a high prevalence of mixed infections. Mol Biochem Parasitol 58 : 283–292. [Google Scholar]
  16. Snounou G, Viriyakosol S, Zhu XP, Jarra W, Pinheiro L, do Rosario VE, Thaithong S, Brown KN, 1993. High sensitivity of detection of human malaria parasites by the use of nested polymerase chain reaction. Mol Biochem Parasitol 61 : 315–320. [Google Scholar]
  17. Snounou G, Zhu XP, Siripoon N, Jarra W, Thaithong S, Brown KN, Viriyakosol S, 1999. Biased distribution of msp1 and msp2 allelic variants in Plasmodium falciparum populations in Thailand. Trans R Soc Trop Med Hyg 93 : 369–374. [Google Scholar]
  18. Wåhlin B, Berzins K, Perlmann H, Anders RF, Perlmann P, 1990. Anti-idiotypic antibodies counteract the invasion inhibition capacity of antibodies to major epitopes of the Plasmodium falciparum antigen Pf155/RESA. Infect Immun 58 : 2815–2820. [Google Scholar]
  19. Bendixen M, Msangeni HA, Pedersen BV, Shayo D, Bødker R, 2001. Diversity of Plasmodium falciparum populations and complexity of infections in relation to transmission intensity and host age: a study from the Usambara Mountains, Tanzania. Trans R Soc Trop Med Hyg 95 : 143–148. [Google Scholar]
  20. Färnert A, Rooth I, Svensson Å, Snounou G, Björkman A, 1999. Complexity of Plasmodium falciparum infections is consistent over time and protects against clinical disease in Tanzanian children. J Infect Dis 179 : 989–995. [Google Scholar]
  21. Farnert A, Snounou G, Rooth I, Bjorkman A, 1997. Daily dynamics of Plasmodium falciparum subpopulations in asymptomatic children in a holoendemic area. Am J Trop Med Hyg 56 : 538–547. [Google Scholar]
  22. Brown GV, Anders RF, Mitchell GF, Heywood PF, 1982. Target antigens of purified human immunoglobulins which inhibit growth of Plasmodium falciparum in vitro. Nature 297 : 591–593. [Google Scholar]
  23. Shi YP, Udhayakumar V, Oloo AJ, Nahlen BL, Lal AA, 1999. Differential effect and interaction of monocytes, hyperimmune sera, and immunoglobulin G on the growth of asexual stage Plasmodium falciparum parasites. Am J Trop Med Hyg 60 : 135–141. [Google Scholar]
  24. Wilson RJM, Phillips RS, 1976. Method to test inhibitory antibodies in human sera to wild populations of Plasmodium falciparum. Nature 263 : 132–134. [Google Scholar]
  25. Bull PC, Lowe BS, Kortok M, Molyneux CS, Newbold CI, Marsh K, 1998. Parasite antigens on the infected red cell surface are targets for naturally acquired immunity to malaria. Nat Med. 4 : 358–360. [Google Scholar]
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ANTIBODY-MEDIATED IN VITRO GROWTH INHIBITION OF FIELD ISOLATES OF PLASMODIUM FALCIPARUM FROM ASYMPTOMATIC CHILDREN IN BURKINA FASO
The American Journal of Tropical Medicine and Hygiene 68, 728 (2003); https://doi.org/10.4269/ajtmh.2003.68.728
/content/journals/10.4269/ajtmh.2003.68.728
/content/journals/10.4269/ajtmh.2003.68.728
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  • Received : 22 Aug 2002
  • Accepted : 03 Mar 2003

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