Volume 68, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


Balamuthia mandrillaris is an opportunistically pathogenic ameba that causes fatal granulomatous amebic encephalitis (GAE) in vertebrates. Previous phylogenetic analyses that included the sequence of a single nuclear small subunit ribosomal RNA gene (18S or ssu rDNA) from this ameba suggested that is closely related to , another opportunistically pathogenic amebic genus, which includes multiple ssu rDNA genotypes. We tested whether this also is true for The nuclear ssu rDNA from 4 isolates and the mitochondrial ssu rDNA from 7 isolates of have been sequenced. No variation in the nuclear rDNA sequences and low levels of variation in the mitochondrial rDNA were found. Both gene sequences were consistent with a single genotype for . The mitochondrial sequences of are unique and should be useful for development of genus-specific diagnostic probes for use with clinical, environmental, and archived specimens.


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  • Received : 14 Feb 2002
  • Accepted : 20 Jun 2002

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