1921
Volume 68, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

The EG95-based vaccine protects sheep from infection with the dog tapeworm The EG95 encoding gene is a member of a multigene family, several members of which are expressed in the oncosphere, believed to be the target of immunity induced by the vaccine. exhibits extensive intraspecific (strain) variation, and variability of the 95 gene in different isolates of may directly impact the effectiveness of the EG95-based vaccine. We analyzed the 95 gene from collected in Xinjiang, in northwest China, where hydatid disease is hyperendemic. The gene is expressed in oncospheres, protoscoleces, and immature and mature adult worms, and the 95 gene family was shown to comprise two basic sequence types. Very limited sequence variation was evident in the EG95 protein from oncospheres. This high degree of sequence conservation predicts that the vaccine will continue to be effective in China and elsewhere.

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2003-01-01
2017-09-19
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References

  1. Lightowlers MW, Lawrence SB, Gauci CG, Young J, Ralston MJ, Maas D, Health DD, 1996. Vaccination against hydatidosis using a defined recombinant antigen. Parasite Immunol 18 : 457–462.
  2. Lightowlers MW, Lawrence SB, Gauci CG, Young J, Ralston MJ, Maas D, Health DD, 1999. Vaccination trials in Australia and Argentina confirm the effectiveness of the EG95 hydatid vaccine in sheep. Int J Parasitol 29 : 531–534.
  3. Lightowlers MW, Flisser A, Gauci CG, Heath DD, Jensen O, Rolfe R, 2000. Vaccination against cysticercosis and hydatid disease. Parasitol Today 16 : 191–196.
  4. Lightowlers MW, Gauci CG, 2001. Vaccines against cysticercosis and hydatidosis. Vet Parasitol 101 : 337–352.
  5. Heath DD, Lawrence SB, 1996. Antigenic polypeptides of Echinococcus granulosus oncospheres and definition of protective molecules. Parasite Immunol 18 : 347–357.
  6. Chow C, Gauci CG, Cowman AF, Lightowlers MW, 2001. A gene family expressing a host-protective antigen of Echinococcus granulosus. Mol Biochem Parasitol 118 : 83–88.
  7. Thompson RC, Lymbery AJ, Constantine CC, 1995. Variation in Echinococcus: towards a taxonomic revision of the genus. Adv Parasitol 35 : 145–176.
  8. Thompson RCA, Lymbery AJ, 1988. The nature, extent and significance of variation within the genus Echinococcus. Adv Parasitol 27 : 209–258.
  9. Thompson RCA, McManus DP, 2001. Aetiology: parasites and life-cycles. Eckert J, Gemmell MA, Meslin F-X, Pawlowski ZS, eds. WHO/OIE Manual on Echinococcosis in Humans and Animals: a Public Health Problem of Global Concern. Paris: World Organisation for Animal Health and World Health Organisation, 1–19.
  10. Chi P, Zhang W, Zhang Z, Hasyet M, Liu F, Ding Z, Andersen FL, Tolley HD, Schantz PM, 1900. Cystic echinococcosis in the Xinjiang/Uygur Autonomous Region, People’s Republic of China. I. Demographic and epidemiologic data. Trop Med Parasitol 41 : 157–162.
  11. Chi PS, Fan YL, Zhang WB, Zhang ZZ, Alili H, Zhang YL, 1989. The epidemic situation of cystic echinococcosis in China. Xinjiang Agric Sci 1989: 35–38 (in Chinese).
  12. Zhang WB, Zhang ZZ, Alili H, Chi PS, 1994. An investigation of factors influencing the hyperendemic of echinococcosis in Xin-jiang. Chinese J Zoonoses 10 : 204–205.
  13. Osborn PJ, Heath DD, 1982. Immunisation of lambs against Echinococcus granulosus using antigens obtained by incubation of oncospheres in vitro. Res Vet Sci 33 : 132–133.
  14. Rajasekariah GR, Rickard MD, Mitchell GF, 1980. Density-gradient separation of Taenia pisiformis oncospheres. J Parasitol 66 : 355–356.
  15. da Silva CM, Henrique, Ferreira B, Picon M, Gorfinkiel N, Ehrlich R, Zaha A, 1993. Molecular cloning and characterization of actin genes from Echinococcus granulosus. Mol Biochem Parasitol 60: 209–219.
  16. Woollard DJ, Gauci CG, Heath DD, Lightowlers MW, 1998. Epitope specificities and antibody responses to the EG95 hydatid vaccine. Parasite Immunol 20 : 535–540.
  17. Woollard DJ, Gauci CG, Lightowlers MW, 1999. Synthetic peptides induce antibody against a host-protective antigen of Echinococcus granulosus. Vaccine 18 : 785–794.
  18. Wollard DJ, Heath DD, Gauci CG, Lightowlers MW, 2000. Assessment of protective immune responses against hydatid disease in sheep by immunization with synthetic peptide antigens. Parasitology 121 : 145–153.
  19. Lightowlers MW. Gauci CG, 2001. Vaccines against cysticercosis and hydatidosis. Vet Parasitol 101:337–352.
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  • Received : 29 Apr 2002
  • Accepted : 11 Sep 2002

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