1921
Volume 68, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

This study compared a panel of 10 enzyme-linked immunosorbent assays (ELISAs) for the serodiagnosis of Mediterranean visceral leishmaniasis (MVL). The ELISAs were based on either one of the following antigens: crude soluble antigens (SLAs), recombinant (r) antigens (namely: rgp63, rK39, gene B protein, rH2A and rH2B histones proteins, rLACK, rPSA-2, r P20) and purified lipophosphoglycan. Most of the test antigens showed good performance (sensitivity > 85%, specificity > 80%). rK39 and SLA-based ELISA gave the best results in terms of sensitivity (100%) and predictive value of the negative (100%). The best specificity (97%) and the best predictive value of the positive (92%) were obtained with rK39. These results show that several antigens are suitable to design a diagnostic ELISA of MVL. However, recombinant proteins add little to the classic crude SLA, which still represents a very good and less costly alternative.

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  • Received : 26 Feb 2002
  • Accepted : 12 Aug 2002

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