Volume 68, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


It is still unclear to what extent myocarditis-associated, chronic Chagas’ heart disease is due to persisting In the present study, we have analyzed tissue samples from the hearts of three patients with this disease. hybridization provided little evidence for the presence of intact even at sites of strong inflammation. Nevertheless, micromanipulation techniques detected remnants of both kinetoplast DNA and nuclear DNA. DNA was also detected in single macrophages dissected directly from frozen heart tissue sections. Thus, this analysis demonstrates that kinetoplast DNA and nuclear DNA are widely dispersed in the heart tissue, although in low amounts. Since we rarely detected intact parasites during the chronic phase of Chagas’ heart disease, we can exclude heart tissue as a major parasite reservoir.


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  • Received : 12 Jun 2002
  • Accepted : 28 Oct 2002

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