1921
Volume 68, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Wild-caught synanthropic flies were tested for the presence of and on their exoskeletons and in their digestive tracks by fluorescent hybridization and fluorescein isothiocyanate (FITC)–conjugated monoclonal antibody (MAb) against and cell wall epitopes. The levels of were positively correlated with the levels of , indicating a common source of contamination. The majority of oocysts and cysts were potentially viable ( = 80% and = 69%). More cysts occurred on the exoskeleton of the flies than within the digestive tracts; the opposite relationship was observed for . No genotype other than G2 was found to be associated with flies. Because filth flies carry viable oocysts and cysts acquired naturally from unhygienic sources, they can be involved in the epidemiology of cryptosporidiosis and giardiasis. Fluorescent oligonucleotide probes used together with FITC–conjugated MAb represent a convenient and cost-effective technique for rapid and specific identification of human-infectious species of and mechanically transported by flies, and for the assessment of the viability of these pathogens.

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2003-02-01
2017-11-20
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  • Received : 27 Dec 2001
  • Accepted : 09 May 2002

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