1921
Volume 68, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Third-stage larvae (L3) of the human hookworm parasite were cultured from the feces of infected hamsters and frozen for up to 100 days in liquid nitrogen. Upon thawing, viable larvae were recovered and used to inoculate naive hamsters. The larvae recovered from this second group of hamsters were used to inoculate a third group of naive animals, which demonstrated that the originally frozen larvae were successfully maintained for two full generations following thawing. These data suggest that exsheathment, which has previously been reported to be essential for successful cryopreservation, is not necessary for recovery of viable, infectious L3.

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2003-01-01
2017-11-23
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References

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  8. Nolan TJ, Aikens LM, Schad GA, 1988. Cryopreservation of first stage and infective third stage larvae of Strongyloides stercoralis. J Parasitol 74: 387–391.
  9. Hawdon JM, Schad GA, 1991. Long term storage of hookworm infective larvae in buffered saline solution maintains larval responsiveness to host signals. J Helminthol Soc (Wash) 58: 140–142.
  10. Garside P, Behnke JM, 1989. Ancylostoma ceylanicum in the hamster: observations on the host-parasite relationship during primary infection. Parasitology 98: 283–289.
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  • Received : 04 Jun 2002
  • Accepted : 25 Sep 2002

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