Volume 68, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


We investigated the frequencies of single and multiple matings in field-collected female by conducting microsatellite DNA analyses on the sperm contained within their spermatheca. Amplifcation by a polymerase chain reaction (PCR) at four loci allowed the detection of sperm extracts exhibiting more than two alleles per locus, thereby revealing the occurrence of multiple inseminations. Polyandry was found in six of 239 females examined, or 2.5% of the samples. Previous analyses of the molecular form of the sperm and female extracts using a PCR-based diagnostic procedure showed that two of these multiple inseminations involved cross-mating between two chromosomal/molecular forms of Thus polyandry occurred within-form in 1.7% of examined females while other multiple inseminations may be linked to processes of reproductive isolation between forms of .


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  1. Davidson G, 1974. Genetic Control of Insect Pests. London: Academic Press.
  2. Curtis CF, 1985. Genetic control of insect pests: growth industry or lead balloon? Biol J Linn Soc 26: 359–374. [Google Scholar]
  3. Coluzzi M, Sabatini A, Petrarca V, Di Deco MA, 1979. Chromosomal differentiation and adaptation to human environments in the Anopheles gambiae complex. Trans R Soc Trop Med Hyg 73: 483–497. [Google Scholar]
  4. Bryan JH, di Deco MA, Petrarca V, Coluzzi M, 1982. Inversion polymorphism and incipient speciation in Anopheles gambiae s. str. in The Gambia, West Africa. Genetica 59: 167–176. [Google Scholar]
  5. Subbarao SK, Sharma VP, 1997. Anopheline species complexes & malaria control. Indian J Med Res 106: 164–173. [Google Scholar]
  6. Krimbas CB, Powell JR, 1992. Drosophila Inversion Polymorphism. Boca Raton, FL: CRC Press.
  7. Price CSC, Kim CH, Grohlund CJ, Coyne JA, 2001. Cryptic reproductive isolation in the Drosophila simulans species complex. Evolution 55: 81–92. [Google Scholar]
  8. Craig GB, 1967. Mosquitoes: female monogamy induced by male accessory gland substances. Science 156: 1499–1501. [Google Scholar]
  9. Klowden MJ, 1999. The check is in the male: male mosquitoes affect female physiology and behavior. J Am Mosq Control Assoc 15: 213–220. [Google Scholar]
  10. Leahy MG, Craig GB, 1965. Accessory gland substance as a stimulant for oviposition in Aedes aegypti and A. albopictus. Mosq News 25: 448–452. [Google Scholar]
  11. Yeh CC, Klowden MJ, 1990. Effects of male accessory gland substances on the pre-oviposition behaviour of Aedes aegypti mosquitoes. J Insect Physiol 36: 799–803. [Google Scholar]
  12. Klowden MJ, Chambers GM, 1991. Male accessory gland substances activate egg development in nutritionally stressed Aedes aegypti mosquitoes. J Insect Physiol 37: 721–726. [Google Scholar]
  13. Aguade M, 1999. Positive selection drives the evolution of the Acp29AB accessory gland protein in Drosophila. Genetics 152: 543–551. [Google Scholar]
  14. Touré YT, Petrarca V, Traoré SF, Coulibaly A, Maiga HM, Sankaré SF, Sow M, Di Deco MA, Coluzzi M, 1998. The distribution and inversion polymorphism of chromosomally recognized taxa of the Anopheles gambiae complex in Mali, West Africa. Parassitologia 40: 477–511. [Google Scholar]
  15. Favia G, della Torre A, Bagyaoko M, Lanfrancotti A, Sagnon N’F, Touré YT, Coluzzi M, 1997. Molecular identifications of sympatric chromosomal forms of Anopheles gambiae and further evidence of their reproductive isolation. Insect Mol Biol 6: 377–383. [Google Scholar]
  16. Favia G, Lanfrancotti A, Spanos L, Sidén-Kiamos I, Louis C, 2001. Molecular characterization of ribosomal DNA (rDNA) polymorphisms discriminating among chromosomal forms of Anopheles gambiae s.s. Insect Mol Biol 10: 19–23. [Google Scholar]
  17. Tripet F, Touré Y, Taylor CE, Norris DE, Dolo G, Lanzaro GC, 2001. DNA analysis of transferred sperm reveals significant levels of gene flow between molecular forms of the Anopheles gambiae complex. Mol Ecol 10: 1725–1732. [Google Scholar]
  18. Powell JR, Petrarca V, della Torre A, Caccone A, Coluzzi M, 1999. Population structure, speciation, and introgression in the Anopheles gambiae complex. Parassitologia 41: 101–113. [Google Scholar]
  19. Black WC, Lanzaro GC, 2001. Distribution of genetic variation among chromosomal forms of Anophele gambiae s.s.: introgressive hybridization, adaptive inversions, or recent reproductive isolation? Insect Mol Biol 10: 3–7. [Google Scholar]
  20. Post RJ, Flook PK, Millest AL, 1993. Methods for the preservation of insects for DNA studies. Biochem Syst Ecol. 21: 85–92. [Google Scholar]
  21. Walsh PS, Metzger DA, Higuchi R, 1991. Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques 10: 506–513. [Google Scholar]
  22. Scott JA, Brodgon WG, Collins FH, 1993. Identification of single specimens of the Anopheles gambiae complex by the polymerase chain reaction. Am J Trop Med Hyg 49: 520–529. [Google Scholar]
  23. Zheng L, Benedict MQ, Cornel AJ, Collins FH, Kafatos FC, 1996. An integrated genetic map of the African human malaria vector mosquito, Anopheles gambiae. Genetics 143: 941–952. [Google Scholar]
  24. Lanzaro GC, Zheng L, Touré YT, Traoré SF, Kafatos FC, Vernick KD, 1995. Microsatellite DNA and isozyme variability in a west African population of Anopheles gambiae. Insect Mol Biol 4: 105–112. [Google Scholar]
  25. Lanzaro GC, Touré YT, Carnahan J, Zheng L, Dolo GT, Traoré SF, Petrarca V, Vernick KD, Taylor CE, 1998. Complexities in the genetic structure of Anopheles gambiae populations in west Africa as revealed by microsatellite DNA analysis. Proc Natl Acad Sci USA 95: 14260–14265. [Google Scholar]
  26. Giglioli MEC, Mason GF, 1966. The mating plug in anopheline mosquitoes. Proc R Entomol Soc Lond A 41: 123–129. [Google Scholar]
  27. Fuchs MS, Craig GB, Hiss EA, 1968. The biochemical basis of female monogamy in mosquitoes. I. Extraction of the active principle from Aedes aegypti. Life Sci 73: 835–839. [Google Scholar]
  28. Fuchs MS, Craig GB, Despommier DD, 1969. The protein nature of the substance inducing monogamy in Aedes aegypti. J Insect Physiol 15: 701–709. [Google Scholar]
  29. Young ADM, Downe AER, 1987. Male accessory gland substances and the control of sexual receptivity in females Culex tarsalis. Physiol Entomol 12: 233–239. [Google Scholar]
  30. Gillies MT, 1956. A new character for the recognition of nulliparous females of Anopheles gambiae. Bull World Health Organ 15: 451–459. [Google Scholar]
  31. Scarpassa VM, Tadei WP, Kerr WE, 1992. Biology of Amazonian mosquitoes. XVI. Evidence of multiple insemination (polyandry) in Anopheles nuneztovari Gabaldon, 1940 (Diptera: Culicidae). Rev Bras Genet 15: 51–64. [Google Scholar]
  32. Marchand RP, 1984. Field observations on swarming and mating in Anopheles gambiae mosquitoes in Tanzania. Neth J Zool 34: 367–387. [Google Scholar]
  33. Giglioli MEC, 1963. The female reproductive system of Anopheles melas. I. The structure and function of the genital ducts and associated organs. Riv Malariol 42: 149–176. [Google Scholar]
  34. Gwadz RW, Craig GB, 1970. Female polygamy due to inadequate semen transfer in Aedes aegypti. Mosq News 30: 354–360. [Google Scholar]
  35. Mahmood F, Reisen WK, 1980. Anopheles culicifacies: the occurrence of multiple insemination under laboratory conditions. Entomol Exp Applic 27: 69–76. [Google Scholar]
  36. Charlwood DM, Jones DR, 1979. Mating behaviour in the mosquito, Anopheles gambiae s.l. 1. Close range behaviour. Ecol Entomol 4: 111–120. [Google Scholar]
  37. Goma LKH, 1963. Tests for multiple insemination in Anopheles gambiae Giles. Nature 197: 99–100. [Google Scholar]
  38. Gomulski L, 1990. Polyandry in nulliparous Anopheles gambiae mosquitoes (Diptera, Culicidae). Bull Entomol Res 80: 393–396. [Google Scholar]
  39. Chapuisat M, 1998. Mating frequency of ant queens with alternative dispersal strategies, as revealed by microsatellite analysis of sperm. Mol Ecol 7: 1097–1105. [Google Scholar]
  40. Gertsch PJ, Fjerdingstad EJ, 1997. Biased amplification and the utility of spermatheca-PCR for mating frequency studies in Hymenoptera. Hereditas 126: 183–186. [Google Scholar]
  41. Gentile G, Slotman M, Ketmaier V, Powell JR, Caccone A, 2001. Attempts to molecularly distinguish cryptic taxa in Anopheles gambiae s.s. Insect Mol Biol 10: 25–32. [Google Scholar]
  42. Wang R, Zheng L, Touré Y, Dandekar T, Kafatos F, 2001. When genetic distance matters: measuring genetic differentiation at microsatellite loci whole genome scans of recent and incipient species. Proc Natl Acad Sci USA 98: 10769–10774. [Google Scholar]
  43. Klowden MJ, 2001. Sexual receptivity in Anopheles gambiae mosquitoes: absence of control by male accessory gland substances. J Insect Physiol 47: 661–666. [Google Scholar]
  44. Bryan JH, 1968. Results of consecutive matings of female Anopheles gambiae species B with fertile and sterile males. Nature 218: 489. [Google Scholar]
  45. Bryan JH, 1972. Further studies on consecutive matings in the Anopheles gambiae complex. Nature 239: 519–520. [Google Scholar]
  46. Bryan JH, 1973. Studies on the Anopheles punctulatus complex. III. Mating behavior of the F1 hybrid adults from crosses between Anopheles farauti no. 1 and An. farauti no. 2. Trans R Soc Trop Med Hyg 67: 85–91. [Google Scholar]
  47. Edillo FE, Touré YT, Lanzaro GC, Dolo G, Taylor CE, 2002. Spatial distribution of Anopheles gambiae and Anopheles arabiensis (Diptera: Cilucidae) in Banambani village, Mali. J Med Entomol 39: 70–77. [Google Scholar]
  48. Baimai V, Green CA, 1987. Monandry (monogamy) in natural populations of anopheline mosquitoes. J Am Mosq Control Assoc 3: 481–484. [Google Scholar]
  49. Yuval B, Fritz GN, 1994. Multiple mating in female mosquitoes -Evidence from a field population of Anopheles freeborni (Diptera, Culicidae). Bull Entomol Res 84: 137–140. [Google Scholar]
  50. Novikov YM, 1981. Monogamy of Anopheles messeae under natural conditions. Zh Zool 60: 214–220. [Google Scholar]
  51. French WL, Kitzmiller JB, 1963. Tests for multiple fertilization in Anopheles quadrimaculatus. Proc N J Mosq Exterm Assoc 50: 374–380. [Google Scholar]

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  • Received : 14 Jan 2002
  • Accepted : 10 Apr 2002

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