1921
Volume 68, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

We investigated the frequencies of single and multiple matings in field-collected female by conducting microsatellite DNA analyses on the sperm contained within their spermatheca. Amplifcation by a polymerase chain reaction (PCR) at four loci allowed the detection of sperm extracts exhibiting more than two alleles per locus, thereby revealing the occurrence of multiple inseminations. Polyandry was found in six of 239 females examined, or 2.5% of the samples. Previous analyses of the molecular form of the sperm and female extracts using a PCR-based diagnostic procedure showed that two of these multiple inseminations involved cross-mating between two chromosomal/molecular forms of Thus polyandry occurred within-form in 1.7% of examined females while other multiple inseminations may be linked to processes of reproductive isolation between forms of .

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2003-01-01
2017-11-22
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  • Received : 14 Jan 2002
  • Accepted : 10 Apr 2002

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