image of Interferon-γ and IL-10 Release Assay for Patients with Ocular Toxoplasmosis
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


Peripheral blood mononuclear cells (PBMCs) from patients with ocular toxoplasmosis were challenged with lysate antigen (TLA) in a cytokine release assay (CRA), run during the inactive period of the disease. Increased interferon gamma (IFN-γ) levels were detected after PBMC stimulation with either ME49 reference strain ( = 0.0015) or local TgCkAr-11-9 isolate ( = 0.0012), as compared with those recorded under basal conditions. TLA from TgCkAr11-9 isolate induced a higher release of IFN-γ than ME49 strain CRA from all tested patients ( = 0.02). The median value of IFN-γ release on TgCkAr-11-9 stimulation (26.03 pg/mL) allowed the classification of patients into high– or low–/non–IFN-γ releasers. Clinical correlations were established with both groups. The results obtained in this study suggest the need to include local strains when performing CRA with TLA.


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  • Received : 16 Feb 2020
  • Accepted : 31 Jul 2020
  • Published online : 08 Sep 2020
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