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- ISSN: 0002-9637
- E-ISSN: 1476-1645
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Financial support: This work was supported by the Universidad Católica de las Misiones grant #141-20160212-104-UCAMI 2015 and by private donations made by “Fundación Bronislada Kruchowski de Szychowski” and Don Basilio SRL.
Authors’ addresses: Marcelo Rudzinski, Silvana Carolina Duarte, and Silvia Reina, Centro de Investigación, Facultad de Ciencias de la Salud, Universidad Católica de las Misiones, Posadas, Argentina, E-mails: [email protected], [email protected], and [email protected]. Lais Pardini, Mariana Bernstein, and Gastón Moré, Facultad de Ciencias Veterinarias, Laboratorio de Inmunoparasitología, Universidad Nacional de La Plata, La Plata, Argentina, E-mails: [email protected], [email protected], and [email protected]. Marina Khoury, Dirección de docencia e Investigación, Instituto de Investigaciones Médicas Alfredo Lanari, Ciudad Autónoma de Buenos Aires, Argentina, E-mail: [email protected]. Carina Argüelles, Cátedra de Biología Molecular, Universidad Católica de las Misiones, Posadas, Argentina, E-mail: [email protected]. José Raúl Oubiña, Instituto de Investigaciones en Microbiología y Parasitología Médica, Universidad de Buenos Aires - Consejo Nacional de Investigaciones Científicas y Técnicas (IMPaM, UBA-CONICET), Buenos Aires, Argentina, E-mail: [email protected].
††These authors contributed equally to this work.
- The American Society of Tropical Medicine and Hygiene
- Source: The American Journal of Tropical Medicine and Hygiene, Volume 103, Issue 6, 2 Dec 2020, p. 2239 - 2243
Interferon-γ and IL-10 Release Assay for Patients with Ocular Toxoplasmosis
Abstract
Abstract.
Peripheral blood mononuclear cells (PBMC) from patients with ocular toxoplasmosis were challenged with total antigens from Toxoplasma gondii lysate (TATL) in a cytokine release assay (CRA), run during the inactive period of the disease. Increased interferon gamma (IFN-γ) levels were detected after PBMC stimulation with either ME49 reference strain (P = 0.0015) or local TgCkAr-11-9 isolate (P = 0.0012), as compared with those recorded under basal conditions. TATL from TgCkAr11-9 isolate induced a higher release of IFN-γ than ME49 strain in CRA from all tested patients (P = 0.02). The median value of IFN-γ release on TgCkAr-11-9 stimulation (26.03 pg/mL) allowed the classification of patients into high– or low–/non–IFN-γ releasers. Clinical correlations were established with both groups. The results obtained in this study suggest the need to include local strains when performing CRA with TATL.
© The American Society of Tropical Medicine and Hygiene
- 16 Feb 2020
- 31 Jul 2020
- 08 Sep 2020