1921
Volume 56, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645
USD

Abstract

Abstract

The relationships between the parasitic nematodes of medical importance belonging to the genus was studied using a polymerase chain reaction (PCR)-linked restriction fragment length polymorphism approach. We used several human and dog isolates of , a monkey isolate of , and , a rodent parasite. The molecular analysis was based on amplification of the internal transcribed spacer and the 5′ portion of the 23S-like rRNA gene followed by restriction enzyme digests. The length of the PCR product was specific to each species and varied around 1.5 kilobase pairs. Using nine restriction enzymes, we were able to analyze both interspecific and intraspecific variations. With four restriction enzymes ( I, I, I, and I), human isolates of from different parts of the world showed identical patterns and could be differentiated from the dog isolate of . Interspecific differences were readily observed with these and other enzymes. In addition to providing new information on the genomic characteristics of parasites, the results suggest that this technique could be useful for diagnostic and epidemiologic investigations.

Loading

Article metrics loading...

/content/journals/10.4269/ajtmh.1997.56.61
1997-01-01
2017-11-24
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.1997.56.61
Loading

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error