Volume 56, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Using an indirect immunofluorescence assay on stool samples, we found a 6.4% prevalence of cryptosporidiosis among 403 children less than five years of age with acute diarrhea in Mexico City over a one-year period. The prevalence was highest (11.4%) during the rainy summer months. Most cases occurred in infants less than one year of age. was more common in malnourished children ( < 0.05) and in nonbreast-fed infants less than six months of age ( < 0.01). Neither dwelling characteristics nor the presence of domestic animals at home were associated with infection. Enteropathogenic bacteria were found in 26.8% of the children; , and being the most frequently identified. None of 100 serum samples tested showed antibodies against human immunodeficiency virus. When compared with immunofluorescence, the acid-fast technique showed a sensitivity of 76.9% and a specificity of 98.9%, with a predictive positive value of 83.3%. It was concluded that 1) monoclonal antibody-based immunofluorescence is a better diagnostic tool than the acid-fast technique, 2) the prevalence of cryptosporidiosis in this population was similar to that of other developing countries, 3) clinical manifestations were nonspecific, and 4) was more common in malnourished children and in nonbreast-fed infants.


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