1921
Volume 55, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645
USD

Abstract

Abstract

Dinucleotide microsatellites were characterized from , a species of mosquito that transmits malaria. A partial genomic library of , consisting of 3,960 kilobases (kb), was screened with either (GT) or (CT) probes. Approximately 1.5% of the recombinants contained sequences that hybridized to either (GT) or (CT) dinucleotide probes, suggesting that microsatellites are abundant in the genome of . Estimation of abundance of the two dinucleotide repeats revealed that (GT) or (CA) microsatellites occur on average every 68 kb and (CT) or (GA) repeats every 495 kb. Among 23 microsatellite loci sequenced, four loci were selected to synthesize primers to perform polymerase chain reaction scoring for genetic polymorphism in a population of . A high level of polymorphism was observed with all four microsatellite loci analyzed. The number of alleles detected at each locus ranged from eight to 12 and the heterozygosities ranged from 0.25 to 0.54. A total of 42 alleles were found among four microsatellite loci. The large number of alleles and polymorphic nature resolved from microsatellite loci make these markers valuable for the study of population genetic structure and gene flow. Knowledge of gene flow is required to develop vector control strategies using genetic manipulations of malaria vector populations.

Loading

Article metrics loading...

/content/journals/10.4269/ajtmh.1996.55.589
1996-12-01
2017-09-22
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.1996.55.589
Loading

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error