1921
Volume 54, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645
USD

Abstract

Abstract

Eukaryotic and prokaryotic cells undergo an increase in heat shock proteins, including hsp70, during exposure to environmental stress and during some developmental changes. In trypanosomatid protozoa such as sp. that cycle between poikilothermic vectors and mammalian hosts, this heat shock response occurs at programmed times in the parasite's life cycle. The increase in heat shock proteins in mammalian cells is initiated by an increased rate of transcription, resulting in greater amounts of total hsp70 RNA and protein. In contrast, we found a dramatic increase in hsp70 RNA during growth of promastigotes from logarithmic to stationary phase in liquid culture, which was not accompanied by an increased amount of hsp70 protein. Furthermore, there was a 1.8-fold increase in hsp70 protein induced by exposure of to superoxide, but this was not associated with an increase in hsp70 RNA. We conclude that in contrast to higher eukaryotes, the amount of hsp70 protein produced by sp. is not regulated by the steady state level of total hsp70 RNA.

Loading

Article metrics loading...

/content/journals/10.4269/ajtmh.1996.54.471
1996-05-01
2017-11-23
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.1996.54.471
Loading

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error