Volume 54, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Genomic DNA probes were made for two recently identified members of the complex; sp. near from Papua New Guinea and No. 7 from the Solomon Islands. The probes are species-specific and with the use of P labeling sensitive enough so that a squash blot of only a small segment of the mosquito is required for identification. The 119-basepair (bp) probe for sp. near and the 1,106-bp probe for No. 7 have been sequenced in full and the probes have been tested on field collected specimens. These probes now make it possible to distinguish sp. near and No. 7 from the other eight members of the complex. A pan-species probe was also made from the 18S ribosomal DNA that binds to DNA from all members of the complex. These three probes complete the set required for distinguishing all known members of the complex by DNA hybridization.


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