Volume 54, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



We have developed a highly sensitive method for detection of in clinical samples based on a nested polymerase chain reaction (PCR) for the gene. Mice infected with were killed at 24-hr intervals, and the DNA from blood and spleens was extracted by a variety of methods and analyzed by PCR. The best method, based on the ability of DNA to bind to silica in the presence of guanidine thiocyanate, yielded amplifiable DNA without dilution of the murine tissues samples. in infected murine spleens and culture-positive blood samples was reliably detected by nested PCR following this extraction procedure. We believe this technique has significant advantages over traditional methods for diagnosing infection in terms of speed, ease of use, reproducibility, and safety.


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