Volume 53, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



A method has been developed to identify the members of the complex using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Members of the complex are the most important vectors of malaria in the southwest Pacific and consist of 10 cryptic species, no. 1–7, sp. near , and . For each species, PCR amplification of the ribosomal DNA internal transcribed spacer produced a 750-basepair product. Digestion with I and electrophoresis on a 3.0% agarose gel results in banding patterns unique to each species. Isolates of the same species from different locations gave an identical pattern. The technique is sensitive enough so that a PCR-RFLP can be generated from as little as a single mosquito leg, allowing the rest of the mosquito to be used for other important epidemiologic analyses such as determining host feeding source, and for parasite detection.


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