1921
Volume 53, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645
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Abstract

Abstract

In patients with cutaneous leishmaniasis in areas of transmission, ulcers may heal without therapy. In the present study, we evaluated the T cell responses of 10 subjects who two years earlier had a rapidly (less than three months) self-healing cutaneous disease. The immunologic responses of these cases were determined by intradermal skin test, measurements of antibodies, lymphocyte proliferative responses, and interferon-γ (IFN-γ) production in cultures stimulated with antigens. These data were compared with those observed in 10 other patients with active cutaneous and mucosal leishmaniasis. Evidence of strong lymphocyte blastogenesis and IFN-γ production was observed in eight of 10 patients with self-healing cutaneous leishmaniasis, with stimulation indices ranging from 32 to 506, and IFN-γ levels ranging from 500 to 2,900 pg/ml. The mean ± SD stimulation index of the lymphocyte proliferative responses (288 ± 247) and the mean ± SD of IFN-γ production after stimulation with antigen (970 ± 960 pg/ml) in subjects with self-healing cutaneous leishmaniasis were similar ( > 0.05) to those observed in patients with mucosal disease (stimulation index = 308 ± 282 and IFN-γ level = 838 ± 819 pg/ml). These responses were higher ( < 0.01) than those observed in patients with active cutaneous leishmaniasis (stimulation index = 50 ± 82 and IFN-γ level = 264 ± 336 pg/ml). The mean ± SD induration of skin test reactions in subjects with self-healing cutaneous leishmaniasis (23 ± 14 mm) and in patients with mucosal disease (18 ± 6 mm) were greater ( < 0.01) than those observed in patients with active cutaneous leishmaniasis (10 ± 6 mm). In contrast with these parameters of T cell response, the mean ± SD absorbances of the enzyme-linked immunosorbent assay to detect antibodies against in patients with self-healing cutaneous leishmaniasis (0.017 ± 0.008) was lower ( < 0.05) than those observed in patients with active cutaneous leishmaniasis (0.067 ± 0.014) or in patients with mucosal leishmaniasis (0.104 ± 0.056). These data indicate that subjects with self-healing cutaneous leishmaniasis have a strong T cell response to leishmania antigens. This response may have been involved in control of their infection.

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/content/journals/10.4269/ajtmh.1995.53.273
1995-09-01
2017-09-23
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