Volume 51, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



A genomic library constructed in a bacteriophage lambda replacement vector (EMBL3) with DNA partially digested with 3A I was screened with P-labeled total DNA, and a strongly reactive recombinant, EMBL3Wb34, was isolated. This clone contained an approximately 16-kb insert that showed some cross-hybridization with and DNA. However, a 969-bp subclone from EMBL3Wb34, designated pWb12, hybridized only with DNA and was able to detect as little as 300 pg. Furthermore, pWb12 could detect DNA from a single infective larva or one microfilaria. It has a moderate copy number (450–700) and appears to be interspersed within the parasite genome. The nucleotide sequence contains 66% A + T and 34% G + C and shows no notable internal repeats.


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