1921
Volume 51, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645
USD

Abstract

Abstract

Monoclonal antibodies directed against the 260-kD galactose-inhibitable adherence protein (GIAP) of inhibit binding of amebic trophozoites to purified colonic mucins, suggesting that anti-GIAP secretory immunoglobulin A (sIgA) may have a role in host defense in invasive amebiasis. We determined by enzyme-linked immunosorbent assay (ELISA) whether a salivary anti-GIAP sIgA response was present in patients from the Republic of South Africa with invasive infection. In 13 patients with amebic liver abscess (ALA), salivary anti-GIAP sIgA was significantly higher (mean ± SD optical density [OD] = 0.448 ± 0.258) than that determined for seven South African adult patients hospitalized with nonamebic illness (0.084 ± 0.072; = 0.002), seven healthy South African Adults (0.194 ± 0.119; = 0.025), and seven healthy adults from Charlottesville, Virginia (0.036 ± 0.023; = 0.004). Of the patients with ALA, nine had acute disease, and four had been cured of amebiasis 2–8 months previously. There was no significant difference between these two groups in the anti-GIAP sIgA levels. All ALA patients had a high titer serum anti-amebic antibody response, and there was no direct correlation between the level of anti-GIAP salivary IgA and anti-GIAP serum antibodies (R = 0.187). These findings demonstrate that the GIAP is a mucosal antigen in naturally occurring invasive infection.

Loading

Article metrics loading...

/content/journals/10.4269/ajtmh.1994.51.454
1994-10-01
2017-09-23
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.1994.51.454
Loading

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error