Volume 51, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



We have shown in a previous study that immunization of a rhesus monkey () with inactivated liver stages of the simian malaria parasite (B strain) produced high antibody titers against sporozoites, liver stages, and blood stages of . In the present study, we demonstrate that these anti- immune sera recognized (Salvador I) antigens. In an indirect immunofluorescence assay, both postimmunization and postchallenge sera reacted with antigens of sporozoite, liver-, and blood-stage parasites. In Western blot analysis, postimmunization sera recognized four bands of 97, 93, 70, and 65 kD in sporozoite antigens; postchallenge sera further recognized three doublet (set of two) bands of 86–90, 73–78, and 44–52 kD. When blood-stage extracts were used as antigens, five bands of 118, 105, 76, 68, and 47 kD reacted with postimmunization sera; two doublet bands of 81–87 and 49–52 kD further reacted with postchallenge sera. None of these sera reacted with asexual blood-stage extracts of and , or with a recombinant circumsporozoite protein of .


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