Volume 51, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Recently described enzyme-linked immunosorbent assay (ELISA) and immunoblot methods for the detection of serum IgG against larval antigens were prospectively evaluated for the diagnosis of endemic strongyloidiasis. A modification of the ELISA involved preincubation of sera with phosphate-buffered saline-soluble extract to remove cross-reactivity with other helminths. The sensitivity of the ELISA increased from 80% to 85% following preincubation. Similarly, there was an increase in specificity from 94% to 97%. The IgG recognition of 41-, 31-, and 28-kD filariform larval components showed sensitivities of 100%, 85%, and 65%, respectively. The specificities of recognition of these proteins were 94%, 89%, and 75%, respectively. Both the ELISA following incubation of sera with extract and serum IgG reactivity to a 41-kD larval component using immunoblotting are sensitive and specific techniques for diagnosing endemic strongyloidiasis.


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