Volume 50, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Current diagnosis of infection requires the direct microscopic identification of the parasite, a technique that is insensitive and cannot distinguish pathogenic from noninvasive . Enzyme-linked immunosorbent assay (ELISA) antigen detection tests were developed to distinguish from infection in stool specimens. The ELISA result for antigen was positive in 26 of 27 -positive stool specimens, three of 25 -positive stools, and one of 30 stools with other or no intestinal parasites, giving a specificity and sensitivity for the detection of infection of 93% and 96%, respectively. The assay result used to detect both and was positive in 26 of 27 -positive stools, 19 of 25 -positive stools, and one of 30 stools negative by microscopy and culture for , giving a specificity and sensitivity of 97% and 87%, respectively. Because these ELISAs can be completed in several hours, they offer promise as rapid and sensitive means of detecting amebic infection.


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