1921
Volume 50, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645
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Abstract

Abstract

The gene coding for the envelope (E) glycoprotein of dengue-2 virus was cloned into baculovirus ( nuclear polyhedrosis virus). The recombinant virus contained the entire E protein gene, preceded by 38 nucleotides from the end of the prematrix glycoprotein gene and followed by the first 83 nucleotides of nonstructural protein 1. When expressed in (Sf9) cells, approximately 1 mg of recombinant E antigen was made per 10 cells. This antigen reacted with polyclonal, antidengue type-2 antibody and a dengue type-2-specific, neutralizing monoclonal antibody. BALB/c mice immunized with the recombinant antigen produced only non-neutralizing antibody against dengue-2 virus, but were partially protected against morbidity and mortality after intracranial challenge with virulent dengue-2 virus.

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/content/journals/10.4269/ajtmh.1994.50.322
1994-03-01
2017-11-22
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