Volume 49, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



BALB/cBYJ mice were immunized against larval by subcutaneous injection of normal, irradiated, or freeze-thaw-killed sp. larvae. The mice received challenge infections of third-stage larva (L) contained in diffusion chambers implanted subcutaneously. At two-weeks postinfection, the diffusion chambers were removed and larval survival was assessed. When mice were immunized a single time with 35-krad-irradiated or normal L, there was a significant reduction in the survival of challenge parasites. However, there was little or no reduction in challenge worm survival when mice were immunized a single time with freeze-thaw-killed L or fourth-stage larva (L), or irradiated L. When a second dose of freeze-thaw killed L or irradiated L was administered, there was a significant reduction in parasite survival in immunized mice. Immunization with L or a combination of L and L failed to confer protection. These results demonstrate that mice can be immunized against larval and that diffusion chambers are an efficient method for studying protective immunity to this parasite in a mouse model.


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