Volume 49, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Polymerase chain reaction primers specific for were used to amplify DNA from extracts of pooled ticks. Amplification was performed on extracts from 140 pools (1,579 total ticks) consisting of three tick genera collected from five states. The characteristic 389-basepair product was observed after amplification of extracts from seven different pools of adult (117 pools, 1,462 ticks), but not from pools of nymphs. No specific product was observed after amplification of 20 pools (105 ticks) of and three pools of (12 ticks). was present in at a minimum frequency of ≥ 0.48%, suggesting that may be a vector of human ehrlichiosis.


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