Volume 48, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



malaria is prevalent during the rainy season in the central highlands of Madagascar. In April 1991, we investigated the cellular and antibody immune responses of 53 inhabitants of Manarintsoa, a village in this area, to four antigens corresponding to B and T cell epitopes of the circumsporozoite (CS) protein. Cellular responses were assessed by lymphocyte proliferation assay as well as by detection of interferon-gamma and interleukin-2 production in vitro. Cell culture was performed with two overlapping synthetic peptides (CSVTCGVGVRVRSRVNA [amino acids 311–326]) and VRVRSRVNAANKKPED [amino acids 319–334]) from the vicinity of the highly conserved region II of the CS protein. In at least one of the three assays, cells from seven subjects showed a positive response to CSVTCGVGVRVRSRVNA, while cells form 14 subjects responded to VRVRSRVNAANKKPED. Antibodies directed against the two recombinant antigens, NS1V20 and rPvCS-2, both of which contain the entire central repeat region of the CS protein, plus regions I and II in the case of rPvCS-2, were measured by the Falcon® assay screening test-enzyme-linked immunosorbent assay. Eight and nine subjects had antibodies to NS1V20 and rPvCS-2, respectively. The presence of antibody responses to both recombinant antigens was related ( = 0.02, by Fisher's exact test), but was not related to the presence of a cellular response to peptides from vicinity of region II ( > 0.1, by Fisher's exact test). These data show that most of the antibody response is probably directed towards the repeat region of the CS protein of , but that the vicinity of region II is able to induce both antibody and cellular responses in some individuals.


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