1921
Volume 48, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645
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Abstract

Abstract

A λgt11 cDNA library was previously constructed from poly(A) RNA extracted from adult worms. A cDNA encoding glutathione S-transferase (FhGST) (GenBank Accession Number M93434) was cloned by screening the library with a rabbit anti-FhGST antiserum. The FhGSTs are suspected to be early developmentally expressed-proteins that act as potent immunogens and may be useful as candidate vaccines against the parasite. The cDNA was sequenced and contains 627 translated bases encoding a 24,211-Dalton polypeptide with 209 amino acids and an isoelectric point (pI) of 5.324. This polypeptide has significant homology with 26-kD GSTs from (57.42%) and (57.14%). In addition, we have identified the predicted T cell epitopes in the FhGST protein molecule.

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/content/journals/10.4269/ajtmh.1993.48.457
1993-03-01
2017-11-21
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