1921
Volume 48, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645
USD

Abstract

Abstract

Pulsed-field gel electrophoresis (PFGE) has allowed karyotypic characterization of protozoa at a species and a subspecies level. Different methods of PFGE have been applied to analysis of isolates from the major morphologic groups and of isolates from the group. We developed a four-day method of contour-clamped homogeneous electric field PFGE that produced reliable chromosomal band separation for isolates from the group. When a significant waterborne outbreak of giardiasis occurred in a town in British Columbia, this biotyping method was assessed by characterization of isolates associated with the outbreak. Results were compared with karyotypes obtained from isolates retrieved in other parts of the province, as well as with reference isolates. Isolates in this study were found to have 4–6 chromosomal bands estimated to be between 1 and 4 Mb in size. The chromosomal complement of the outbreak-associated isolates was relatively homogeneous and distinguishable from the non-outbreak-associated British Columbia and reference isolates. The present study showed this PFGE method to be useful in biotyping isolates of . This study also exemplifies the degree of heterogeneity displayed at the genotypic level of , as well as the apparent stability of the outbreak-associated karyotype during transmission from original infecting animal through a water vehicle to human cases.

Loading

Article metrics loading...

/content/journals/10.4269/ajtmh.1993.48.134
1993-01-01
2017-11-18
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.1993.48.134
Loading

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error