Volume 46, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



A field study was initiated in 1988 to investigate whether spotted fever group rickettsiae occur in geographic areas in Egypt that are adjacent to an area in the southern Israeli Negev that has a defined focus of spotted fever disease. Ticks were collected from dogs, sheep, and camels at four study sites in the northern Sinai. Tick hemolymph was processed for rickettsial detection by staining with fluorescein isothiocyanate-conjugated antibody to . Of the 442 hemolymphs examined, 15 contained immunofluorescent rickettsiae. Eight hemolymph test-positive (HT +) ticks were removed from dogs; the other HT + ticks comprised three species, , and . Both HT + and HT - ticks were tested for rickettsial DNA using the polymerase chain reaction (PCR). Eight of 10 HT + field-collected ticks were PCR positive (PCR +). All laboratory colony -infected ticks were PCR +. No HT - ticks from field or laboratory isolates were PCR +.


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