Volume 45, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Primary cultures of and monkey hepatocytes were infected with sporozoites of the NF 54 strain from mosquitoes fed on gametocyte cultures, and with sporozoites of the Santa Lucia strain from mosquitoes fed on an infected monkey. After 4–8 days, one exoerythrocytic (EE) parasite per 30,000 sporozoites was detected in one of three experiments performed with the NF54 strain. However, numerous EE parasites were detected in and cells infected with sporozoites of the Santa Lucia strain. At day 6, most of the parasites contained several hundred nuclei, and were morphologically similar to those previously described in vivo using light or electron microscopy. A monoclonal antibody directed against the repeat region of the circumsporozoite protein of labeled the plasma and parasitophorous vacuole membrane of five-day-old EE parasites by immunoelectron microscopy, thus supporting previous observations by immunofluorescence indicating that the CS protein persists throughout the EE development of . These results demonstrate that liver stages of can be obtained in and monkey cells; they also suggest a parasite strain specificity for hepatocytes.


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