Volume 45, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



A cDNA that encodes tropomyosin, except for 10 amino acids at the amino terminus, has been cloned into a pOTSNCO plasmid vector. Induced expression resulted in a constant level of recombinant protein production. The recombinant tropomyosin was purified from preparative SDS-PAGE gel and by a combination of 20% ammonium sulfate fractionation and fast protein liquid chromatographyionexchange chromatography. The purified recombinant tropomyosin was tested as an immunodiagnostic reagent in Western blot and enzyme-linked immunosorbent assays. Sera from individual patients with chronic infection, but not , parasitic infections other than schistosomiasis, and without infection reacted with the recombinant tropomyosin. The species specificity of tropomyosin suggests that further study of its potential as an immunodiagnostic reagent is warranted.


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