1921
Volume 44, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645
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Abstract

Abstract

Ninety-four leishmanial isolates from the Brazilian Amazon Region (Amapá, Amazonas, Pará, and Rondônia) were identified and classified using specific monoclonal antibodies and an indirect radioimmunoassay (serodeme analysis); eighty-two were also characterized by enzyme electrophoresis (zymodeme analysis), the results of which were subjected to a numerical phenetic analysis. Six isolates from humans (3), (1), (1), and (1) showed reactivity patterns and isoenzyme profiles similar to those obtained with the reference strains, and were identified as this species. Eighty-six stocks were classified as members of the complex; of these, 61 were or variants, which presented three serodeme subtypes, but whose isoenzyme profiles were all similar to the reference strain. A total of 15 isolates were distinguished as or variants and were classified into five serodeme subtypes. The isolate from appeared, from the numerical analysis, to be a distinct parasite species. Ten isolates showed reactivity patterns and isoenzyme profiles similar to those obtained with the reference strain. A parasite isolated from appeared to be different from all reference strains by both techniques, and was classified as probably being a new species. The importance of these results with respect to the taxonomic status of the New World , and their implications for both clinical and epidemiologic data are discussed.

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/content/journals/10.4269/ajtmh.1991.44.645
1991-06-01
2017-09-21
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