Volume 44, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



More than 28,000 mosquitoes in four genera were collected from high elevation (≥1,000 m) areas of California during 1988–89 and tested for virus by plaque assay in Vero cells. Viruses were serogrouped by enzyme immunoassay and serotyped by cross-neutralization. Six strains of Jamestown Canyon virus in the California serogroup were isolated from three species of boreal in the group of the subgenus . All isolates were from mosquitoes collected in Alpine County at approximately 2,300 m elevation in the Sierra Nevada. These included one virus from a pool of male collected in immature stages, which is evidence for vertical transmission; four viruses from adult female and one virus from adult female . These are the first isolations of viruses from boreal mosquitoes in California and the first reported isolations of Jamestown Canyon virus from or .


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