1921
Volume 44, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645
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Abstract

Abstract

Monoclonal antibody-based enzyme-linked immunosorbent assay and DNA hybridization techniques were developed and evaluated for their potential in the detection of infection in humans. A mixture of three IgG monoclonal antibodies (MAb) specific for the 89 kDa metabolic product of was captured on a microtiter plate by rabbit anti-mouse IgG and used in a sandwich ELISA for the detection of parasite antigen. The 89 kD component bound to the MAb was detected with biotinylated rabbit IgG antibody to metabolic products. As little as 0.05–0.1 ng of the antigen could be detected by this technique. A specific DNA probe constructed from a repetitive DNA segment containing 340 base pairs was used in a dot blot hybridization for the detection of parasite DNA. The labeled probe constructed as such could detect DNA released from as few as five eggs. Both methods were specific for and their sensitivity was comparable with that of the classical parasitological technic.

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/content/journals/10.4269/ajtmh.1991.44.140
1991-02-01
2017-09-20
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