1921
Volume 43, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645
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Abstract

Abstract

We have adapted cells to Eagle's minimal medium containing nonessential amino acids, glutamine, and 5% fetal bovine serum. In this medium, cells maintained at 28°C under a 5% CO atmosphere had a doubling time of 42 hr. Ninety-three percent of the cells in a typical population contained 6 full-size chromosomes and an apparent chromosome fragment. cells could be distinguished from cells by the sizes of the small heat shock proteins, whose synthesis was induced by a brief treatment at 41°C. Analysis of the small heat shock proteins synthesized by dissected ovaries from and confirmed the species of origin of the 2 cell lines.

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/content/journals/10.4269/ajtmh.1990.43.669
1990-12-01
2017-11-25
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