Volume 43, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Sera from 38 Paraguayans with positive skin tests to and 51 sera from Paraguayan patients in different stages of Chagas' disease were analyzed by Western blotting using antigen from Y strain epimastigotes and from promastigotes. Using a single serum sample, distinctive patterns of IgG antibody response to both antigens were identified allowing the differentiation between infection, infection, and probable double infection. Sera from patients with infections consistently recognized bands of ∼25 kDa, 38 kDa, and >97 kDa in antigen lysates and recognized a band of 38 kDa in lysates. Sera from patients with infections recognized a band of 66 kDa in antigen lysates but did not consistently recognize any bands using antigen. Sera from patients with probable double infections recognized all bands normally detected by individual sera from patients infected with either or . In our study population, infection among leishmaniasis patients was as frequent as among individuals free of leishmaniasis.


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