1921
Volume 43, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645
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Abstract

Abstract

Sera from patients with confirmed or suspected trichinellosis were examined for 1 year to detect the presence of parasite-specific antibodies (IgG, IgM, and IgE) using the enzyme-linked immunosorbent assay (ELISA). The indirect ELISA was used to detect specific IgG (ELISA-IgG) and specific IgM (ELISA-IgM); an amplified technique proved the most reliable for detection of specific IgE (a-ELISA-IgE). The immunofluorescence (IF) test was used to detect specific IgG (IF-IgG). The patients were from an outbreak of trichinellosis in Salsomaggiore (northern Italy) in 1986. The parasite was isolated and isoenzymatically typed as sp. 3. The specificity of our tests was >95%. During the 1st period of infection, all tests used gave practically the same positivity rate (78.2–86.9%). One year after infection, ELISA-IgG gave the highest positivity rate (55%). With the other tests, the positivity rate was 20–38.5%. At the 2nd month of infection, the IF-IgG test was the most discriminating in patients with confirmed and suspected trichinellosis, but ELISA-IgG proved the most reliable test for detecting specific immunoglobulins in late human trichinellosis infection.

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/content/journals/10.4269/ajtmh.1990.43.186
1990-08-01
2017-11-21
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