1921
Volume 42, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645
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Abstract

Abstract

Synthetic peptides reproducing 4 DRAGQPAG (D4) and a sequential array of DRAGQPAG and DRAGQPAG repeats (DDAAD) of the circumsporozoite (CS) protein were investigated for their potential use in the detection of sporozoite antibodies in human sera. These peptides specifically inhibited the binding of monoclonal antibodies to the CS protein in Western blots. However, when D4 and DDAAD peptides were used in an enzyme-linked immunosorbent assay (ELISA) for the detection of human antibodies, more sera bound to the DDAAD (61%) than to the D4 peptide (22%). This binding was specific, and suggested that the DDAAD peptide contained epitopes constituted by the sequential array of DRAGQPAG and DRAGQPAG repeat variants and absent in the D4 peptide. The ELISA using the DDAAD peptide was applied to the detection of CS protein antibodies in a large number of sera from Kataragama, an endemic area in Sri Lanka. The prevalence of these antibodies increased with age, reaching 40% in adults > 50 years old. The ELISA employing the DDAAD peptide represents a simple and useful tool for the analysis of the antibody response to sporozoites in naturally exposed individuals.

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/content/journals/10.4269/ajtmh.1990.42.17
1990-01-01
2017-11-25
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