1921
Volume 41, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645
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Abstract

Abstract

Cells from adult were fused with cells from a murine BALB/c myeloma Sp2 line. The hybrid cells were grown in HAT (hypoxanthine, aminopterin, and thymidine) medium, cloned and subcloned, and shown to express parasite antigen for 1 year after fusion. Expression of parasite antigen was demonstrated by the following: 2 histogram flow cytometric analyses, in which a population of hybrid cells in the population of 7 month cultured hybrid cells showed 57% more fluorescence when treated with an anti- serum followed by anti-rabbit immunoglobulin G coupled to fluorescein isothiocyanate as compared with the same hybrid cells washed and treated with normal rabbit serum; Sp2 myeloma cells treated with an anti- serum or normal rabbit serum followed by fluorescein-labeled anti-rabbit IgG had the same negative fluorescence; BALB/c mice immunized with PBS-washed cells from a subclone of these hybridomas developed anti- antibodies (shown by the Falcon assay screening test enzyme-linked immunosorbent assay); and antibodies recognized an antigenic polypeptide of 57,000 M in a Western immunoblot. These helminth:myeloma hybrids expressed murine host markers, further confirming the hybrid nature of this cell line. cells alone, like their Sp2 fusion partners, die in HAT supplemented medium by 9 days of culture. :Sp2 hybridomas have been grown continuously in HAT medium for > 1 year.

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/content/journals/10.4269/ajtmh.1989.41.674
1989-12-01
2017-11-21
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