Volume 39, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Enzyme-linked immunosorbent assays (ELISAs) for the circumsporozoite (CS) antigens of , and were used to identify species of sporozoite and oocyst infections detected by dissection in s.l. and collected in western Kenya. ELISAs identified 92.5% of 1,113 salivary gland infections; species infections included 79.4% , 3.2% , 1.7% , and 2 or more species were detected in 15.7% of the in which the species of parasite was identified. Identification was more likely with greater numbers of sporozoites observed in dissections, increasing from 65% ELISA positivity in mosquitoes with 1–10 sporozoites in their salivary glands to 96% in mosquitoes with over 1,000 sporozoites. ELISAs detected CS antigen in 66% of 294 that by dissection had oocysts but uninfected salivary glands. Of 112 with a single species of detected in the salivary glands, 29 (25.9%) had 1 or more additional species detected in the midgut, indicating a high potential for multiple infections. Similar proportions of species were found in s.l. and .


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