Volume 39, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Twenty-four antigens of , a bacterium which causes bartonellosis in residents of high altitude valleys of the Andes, were identified by immunoblot and immunoprecipitation using rabbit anti- sera as well as sera of patients. The antigens were designated according to their relative molecular mass which ranged from 16 to 160 kDa. Twelve antigens were detected by antibodies in sera of bartonellosis patients using immunoblot, of which six antigens were detected by immunoprecipitation. Antigens 25, 46, 65, 75, 99, and 160 were identified ad probable cell wall antigens. Antigens 50, 65, and 75 detected long-persisting antibodies. Crude antigen applied to ELISA reacted with anti- antibody as well as with antibody of unknown identity in human sera, whereas immunoblot and immunoprecipitation with Triton soluble antigens revealed -specific results. Seven antigens were prepared by high performance liquid chromatography of which one antigen (48 kDa) reacted -specific when applied to ELISA. It was concluded that specificity of antibody determination with crude antigen should be confirmed by either immunoblot or immunoprecipitation.


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