1921
Volume 38, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645
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Abstract

Abstract

The serologic activity of a cationic fraction (denoted CEF6) of soluble egg antigen was compared in an ELISA with that of the unpurified soluble egg antigen for the ability to detect human infections and for the prediction of chemotherapeutic success in patients followed up to 5 years post-treatment with oxamniquine. The cationic fraction correctly identified 100% of 20 patients as infected with ; moreover, 50% (10 of 20) seroconverted to negative by 2 years post-treatment and 100% of 15 patients tested were negative 5 years post-treatment. In general, the cationic fraction was superior to the unpurified soluble egg antigen for the detection of infection and for the prediction of chemotherapeutic success. The cationic fraction also exhibited greater immunologic specificity over the unpurified soluble egg antigen in that the latter exhibited higher titers than the former to antibodies against heterologous parasite antigens ( adult worms; larvae). Moreover, rabbit antisera raised against egg antigens isolated from the precipitation formed when fresh eggs are incubated with infection of immunization sera (known as circumoval precipitin reactions or COP) reacted strongly with the cationic fraction in ELISA. In addition, antiserum to the cationic fraction as well as antisera against either of the two antigenic components of this fraction, known as antigens α and θ, all give positive COP reactions when incubated with fresh eggs. This suggests that the cationic fraction antigens are a subcomponent of the COP reaction, a phenomenon which has been used as a highly sensitive and specific serological assay for the detection of infections.

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/content/journals/10.4269/ajtmh.1988.38.508
1988-05-01
2017-09-23
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http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.1988.38.508
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  • Accepted : 28 Oct 1987

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