1921
Volume 37, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645
USD

Abstract

Abstract

Upon exposure to antigen, lymphocytes from patients treated for amebic liver abscess produce lymphokines which activate monocyte-derived macrophages to kill trophozoites. We now demonstrate that gamma interferon (IFN-γ) is produced by these stimulated lymphocytes and is sufficient but not exclusively necessary to activate monocyte-derived macrophage amebicidal activity. Supernatants from mononuclear cells of 7 patients when stimulated with amebic antigen contained more IFN-γ than comparable supernatants derived from control cells (1,862 U/ml vs. 174 U/ml geometric means, < 0.01); IFN-γ levels were similar in patient and control supernatants following concanavalin A stimulation. Macrophages activated solely by partially purified IFN-γ or recombinant human IFN-γ (300 U/ml) killed 47% of virulent amebae by 6 hr at 37°C. Monocyte-derived macrophages stimulated with lymphokines elicited by amebic antigen or concanavalin A killed 48% and 57% of axenic trophozoites, respectively, over 6 hr at 37°C ( < 0.001 for each compared to control). Macrophages incubated with the identical lymphokines, but in the presence of monoclonal antibody to IFN-γ, were only able to kill 18% and 27% of amebae, respectively, at 6 hr ( < 0.05 to control or when antibody to IFN-γ was not present). If antibody to IFN-γ was added to the stimulating lymphokine, more macrophages died during interaction with amebae ( < 0.05). In summary, IFN-γ has a major but not exclusive role in activating human monocyte-derived macrophages in vitro to kill virulent trophozoites.

Loading

Article metrics loading...

/content/journals/10.4269/ajtmh.1987.37.72
1987-07-01
2017-11-18
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.1987.37.72
Loading
  • Accepted : 12 Jan 1987

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error