1921
Volume 37, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645
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Abstract

Abstract.

A genomic library of a savanna isolate of was screened to detect recombinant plasm ids containing highly repeated DNA sequences of this parasite. Four recombinant plasmids were identified which hybridized specifically to DNA, but not to DNA from humans, black flies, , , or . The recombinant plasm ids had a low level of homology to . All recombinant plasmids contain related DNA sequences based on Southern hybridization analysis. Sequences related to these recombinant plasmids are present in different geographic isolates of and , an animal parasite. Two of the recombinant plasmids contain sequences also found in . One recombinant plasmid, puOvs3, has been characterized in detail, including DNA sequence determination. Radiolabeled puOvs3 is able to detect 100 pg of genomic DNA isolated from worms from both savanna and forest regions. It can differentiate from by Southern blot analysis.

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/content/journals/10.4269/ajtmh.1987.37.376
1987-09-01
2017-09-25
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  • Accepted : 09 Mar 1987

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