Volume 37, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Rabbit antisera to 11 isolates were reacted with 8 isolates using single antibody ELISA and indirect immunofluorescent antibody techniques (IFA). Using living trophozoite organisms, IFA showed marked surface fluorescence with homologous antisera-organism pairs while heterologous pairs showed reduced or no reactivity. Using formalin -fixed trophozoites, the pattern of fluorescence changed to include diffuse internal fluorescence with both homologous and heterologous pairs. In contrast to the variability in surface fluorescence, similar reactivity was noted for homologous and heterologous antisera-isolate pairs with the ELISA. In addition, a double antibody enzyme immunoassay using rabbit antisera prepared to 2 isolates was performed with 8 different isolates as test antigen. All 8 were equally well detected. These data confirm previous findings that isolates have both different and common antigens. The different antigens appear to be on the surface of the organism while the common antigens appear to be internal or somatic.


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  • Published online : 01 Sep 1987
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