1921
Volume 37, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645
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Abstract

Abstract.

Soluble antigens from were studied in terms of their ability to react with sera from human visceral leishmaniasis. Thirty-six polypeptides, with molecular weights ranging from 14,400 to 123,000 were demonstrated by Western blot analysis. An extensive cross-reactivity with sera from patients with cutaneous leishmaniasis and Chagas' disease also was observed. Two polypeptides (M 119,000 and 123,000) reacted with all the sera from visceral leishmaniasis patients. When they were electroeluted from gels and evaluated with respect to specificity to the subspecies, these components were expressed in all strains of Leishmania tested, but not in those of . These results indicated that these components are shared by Trypanosomatidae of genus . The eluted polypeptides did not react with sera from patients with Chagas' disease, indicating the feasibility of using purified antigens to discriminate between the humoral immune responses in and infections.

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/content/journals/10.4269/ajtmh.1987.37.263
1987-09-01
2017-09-24
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  • Accepted : 18 Mar 1987

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