1921
Volume 37, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645
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Abstract

Abstract

Transovarial and transstadial passage of was demonstrated for the first time in the western black-legged tick, . One of three field-collected females with spirochetes in ovarial tissues produced 100% infected progeny that maintained the spirochetes transstadially and in 4 of 5 cases passed them via eggs to as many as 97% of F filial ticks. The progeny infected ovarially and by subsequent transstadial passage had generalized tissue infections that exhibited reduced immunofluorescence staining reactivity with a fluorescein isothiocyanate-labeled polyclonal antibody. Attempts to isolate the spirochete from ticks in BSK medium or various modifications of it were unsuccessful. Spirochetes in tissue smears of all three parasitic stages of the F generation were nonreactive with a monoclonal antibody (H5332) specific for , whereas those present in tissue smears of F larvae bound with it.

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/content/journals/10.4269/ajtmh.1987.37.188
1987-07-01
2017-11-19
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http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.1987.37.188
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  • Accepted : 13 Feb 1987

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