1921
Volume 36, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645
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Abstract

Abstract

We report in this paper significant differences in the virulence of insect-derived and cultured metacyclic forms of which are morphologically indistinguishable. Mice infected intraperitoneally with 10 metacyclic isolated from showed average parasitemia levels greater than 2 × 10 organisms/ml around day 10 post-infection (when first measured) and peak levels recorded on day 16 post-infection exceeded 4 × 10 organisms/ml. None of these animals survived after 30 days post-infection. In contrast, in mice infected with 10 or 10 metacyclic forms from axenic cultures the highest average parasitemia was approximately 10 organisms/ml and occurred around day 19 post-infection. In these animals, parasitemias declined with time to become undetectable and no mortality was recorded over the 100-day observation period. There was also a marked difference in the 50% lethal dose of insect- and culture-derived metacyclics. The value for the former was 670 parasites whereas none of the mice infected intraperitoneally with up to 10 cultured metacyclics died. These results point to a marked difference in the biological properties of insect-borne and cultured metacyclics under our experimental conditions and caution against extending results obtained with the latter to vector-transmissible metacyclics, at least in infectivity and virulence studies.

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/content/journals/10.4269/ajtmh.1987.36.529
1987-05-01
2017-09-22
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http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.1987.36.529
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  • Accepted : 16 Oct 1986

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